Ascentis RP-Amide HPLC Columns

Ultra-Low Bleed Alkyl Amide Phase that Rivals C18 as a Generic Scouting Column. Excellent Peak Shape and Resolution, Especially for Polar Compounds or Mixtures of Compound Polarity.

As pioneers in embedded polar group (EPG) phases for HPLC, Supelco is pleased to offer Ascentis RP-Amide, which has all the benefits of enhanced polar compound retention and selectivity, without any of the disadvantages of competitive EPG phases.

Ascentis RP-Amide can be used for many of the same separations as a C18 while avoiding some of the disadvantages of C18 such as poor wettability in high aqueous mobile phases. In addition, it is much more retentive for those molecules that can interact by hydrophobic interactions and also by H-bonding with the amide group. Compared to alkylonly phases, Ascentis RP-Amide has enhanced retention and selectivity for phenols, organic acids and other polar solutes due to strong H-bonding between the amide carbonyl (H-bond acceptor) and H-bond donors, like phenols and acids. Compared to other EPG phases, like carbamates, ureas, sulfonamides and ethers, Ascentis RP-Amide gives retention comparable to C18 and C8 for easy column comparison without the need to change mobile phase conditions.