Ficoll PM 400

CYTIVA

A highly stable sucrose polymer with an average molecular weight of 400 000, suitable for isolating eukaryotic cells and organelles with minimal damage.

For producing density gradients for separation of cells and subcellular components by centrifugation or by sedimentation at unit gravity.
Concentrations of up to 50% (w/v) covering densities of up to 1.2 g/mL can be achieved.
Better osmotic properties than sucrose.
Preserves functional and morphological integrity.
Useful for separating cells that are sensitive to centrifugation and for separating cells of similar density but different size (under conditions of sedimentation at unit gravity).
Does not penetrate biological membranes.
Serves as the raw material for preparation of Ficoll-Paque gradients.
Used in other applications such as electrophoresis, hybridization, cryopreservation, and as a hapten carrier.

Application

Cell isolation

Density Max.

1.2 g/ml

Endotoxin Activity Max.

0.12 EU/mg

Estimated shelf life from manufacture date

5 years

Storage conditions

4–30°C

Stability

Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature

Physical State

Solid

Physical Form

Spray-dried powder

Molecular Weight (Mr) Max.

~ 5 × 10~ 5

Molecular Weight (Mr) Min.

~ 3 × 105

Stokes Radius

~ 10 nm

Color

White

Specific Rotation (alpha)20

50°-65°

Relative Viscosity

5 ηr at 20°C: 10% (w/v) 20 ηr at 20°C: 20% (w/v) 60 ηr at 20°C : 30% (w/v 180 ηr at 20°C : 40% (w/v) 600 ηr at 20°C : 50% (w/v)

Intrinsic Viscosity

~ 17 ml/g

Certificate of Analysis

Yes

17030005	Ficoll PM400	5 kg
17030008	Ficoll PM400	40 kg
17030010	Ficoll PM400 (Dry Powder)	100 gr
17030050	Ficoll PM400 (Dry Powder)	500 gr

Ficoll PM 400

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