ÄKTA Pure Chromatography System

CYTIVA
ÄKTA pure™ is a flexible and intuitive chromatography system for fast purification of proteins, peptides, and nucleic acids from microgram levels to tens of grams of target product. ÄKTA pure chromatography system is a reliable system where hardware and UNICORN™ software are designed to work together with columns and chromatography resins to meet purification challenges.

We offer two versions of ÄKTA pure chromatography system: ÄKTA pure 25, designed for a broad range of research applications and purification tasks in a multiuser environment; and ÄKTA pure 150, created for optimizing resource utilization and productivity in routine large-scale preparative purification. The system supports a wide range of chromatography techniques and meets automation requirements needed to deliver high purity. You can add on to the system at any time with a wide range of options to further increase its capabilities depending on your purification needs.

ÄKTA pure chromatography system is the product of over 50 years of expertise in protein research and three decades of experience in the development of ÄKTA™ purification systems.
  • Application supported : Affinity chromatography, size exclusion chromatography (SEC, also known as gel filtration), ion exchange chromatography, hydrophobic interaction chromatography, and reversed phase chromatography (RPC). For RPC runs use only Frac F9-R or F9-T. 
  • Autosampler injection : Optional
  • Buffer selection : Upgradeable to 14 inlet
  • Compliance : The product fulfills valid directives and standards when used within the conditions specified in the user manual. The product must also be used in the same state as it was delivered from Cytiva. 
  • Dimensions (W × H × D) : 535 × 630 × 470 mm
  • Weight (excluding computer, sample pump, fraction collector) : Up to 53 kg
  • Power consumption : 300 VA (typical), 25 VA (power-save)
  • Protection class : IP 21 
  • System pump type : Piston pump, metering type 
  • Flow rate :  

           ÄKTA pure 25 and ÄKTA pure micro: 0.001 to 25 mL/min (up to 50 mL/min during column packing) 

           ÄKTA pure 150: 0.01 to 150 mL/min (up to 300 mL/min during column packing)

  • Operating Pressure range :

          ÄKTA pure 25 and ÄKTA pure micro: 0 to 20 MPa 

          ÄKTA pure 150: 0 to 5 MPa

  • Multiple sample injection : Upgradeable to 5 samples 
  • Multistep purification : Yes, (optional) 
  • Relative humidity : 20% to 95% (noncondensing) 
  • Safety certifications : CE, ETL, UKCA, EAC, KC, RCM 
  • UV wavelength : 

          UV monitor U9-L : 280 nm

          UV monitor U9-T : 260 and 280 nm fixed

          UV monitor U9-M : 190 to 700 nm in steps of 1 nm, up to three simultaneous wavelengths

  • Viscosity : 0.35 to 10.0 cP (system pump) 
  • Voltage : 100 to 120/220 to 240 VAC 
  • pH monitoring : 0 to 14 (optional) 
  • pH stability, cleaning-in-place (CIP) : 0 to 14 
  • pH stability, operational : 2 to 12